Protein acidification and hydrolysis by pepsin ensure efficient trypsin-catalyzed hydrolysis
نویسندگان
چکیده
Proteolysis of bovine serum albumin by trypsin.
منابع مشابه
Comparative Hydrolysis of Gelatin by Pepsin, Trypsin, Acid, and Alkali
A comparison has been made of the relative velocity of hydrolysis of the various peptid linkings of the gelatin molecule when hydrolyzed by acid, alkali, pepsin or trypsin. It has been found that: 1. Those linkages which are most rapidly split by pepsin or trypsin are among the more resistant to acid hydrolysis. 2. Those linkages which are hydrolyzed by pepsin are also hydrolyzed by trypsin. 3....
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The preparation of novel immobilized and stabilized derivatives of trypsin is reported here. The new derivatives preserved 80% of the initial catalytic activity toward synthetic substrates [benzoyl-arginine p-nitroanilide (BAPNA)] and were 50,000-fold more thermally stable than the diluted soluble enzyme in the absence of autolysis. Trypsin was immobilized on highly activated glyoxyl-Sepharose ...
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Ruan Ch.-Q., Chi Y.-J., Zhang R.-D. (2010): Kinetics of hydrolysis of egg white protein by pepsin. Czech J. Food Sci., 28: 355–363. Taking into account the enzyme inactivation and substrate inhibition, the bioreaction mechanism and kinetics characteristic of egg white protein (EWP) enzymatic hydrolysis by pepsin were investigated. A logarithmic equation h = (1/b) ln (1 + abt) indicating the rel...
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Solid ovalbumin has been irradiated at three doses, 6.5, 25, and 40 megarads, under high vacuum. The native and irradiated samples have been hydrolyzed at pH 1.4 by pepsin, after centrifugation of the aggregates, if necessary. The number of bonds broken per ovalbumin molecule has been estimated by comparing the rate of protein destruction with the rate of formation of NH(2) groups. Both rates i...
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Treatment of swine pepsin with acetylimidazole to acetylate approximately five of its 16 tyrosyl residues causes a significant enhancement of catalytic efficiency (kcat/Km) toward substrates such as dansyl-glycyl-glycyl-L-phenylalanyl-L-phenylalanine 3-(4-pyridyl)propyl ester and benzyloxy-carbonyl-(glycyl)n-p-nitroLphenylalnyl-Lphenylalanyl-L-tyrosine (where n = 0, 1,2). Stopped-flow kinetic s...
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ژورنال
عنوان ژورنال: Food & Function
سال: 2021
ISSN: ['2042-6496', '2042-650X']
DOI: https://doi.org/10.1039/d1fo00413a